Decomposition experiments

Decomposition is part of the nutrient cycle and its rate determines carbon balance of a particular ecosystem. Being an important part of ecological studies, decomposition rate is commonly one of the monitoring programs of field stations. The focus of our research was to estimate the decomposition dynamics in major ecosystems types in middle taiga zone of Western Siberia. The classical approach of litter bags was used, including standard litter type (tea) and native litters in natural as well as in experimental conditions (temperature raise by Open Top Chambers).

MUKHRINO DECOMPOSITION DATABASE could be downloaded and used upon contact (last update 24 Fabruary 2020).

Totally 2657 bags (native and standard litters) were installed by November 2019. To date, totally 1389 bags have been extracted and the data about mass loss are available in the database by February 2020.

The data of this project were also uploaded in DEIMS database, where they could be retrieved.

The extracted litters are stored in zip-lock bags (dried) and available for further analyses and studies of chemical and organic content, litter quality, etc.

The extracted litter bags were photographed (general view of extracted bags and view of extracted from the bags litters under macro-lens) and these pictures were numbered according to sequential bags numbers and are stored as additional information to the database. Totally there are about 100 pictures which could be used for information about extracted litter quality, roots and mycelium content. Link to the collection of pictures.


  • The decomposition rate studies were initiated in 2010 in Mukhrino Field Station (and several years earlier in other locations in vicinity of Khanty-Mansiysk) by the Institute of Soil Science and Agrochemistry (Novosibirsk), these data are currently unavailable to us but could be found upon personal contact.

The decomposition rate experiments by MFS  were started in 2016 as follows:

  • The first series of experiments was initiated with TeaComposition initiative (Djukic et al., 2018) where standard green and roiboos teas were used to estimate decomposition rates in three vegetation types for 4 years.
  • The series of native litter types (totally 11 types, for 4 years of extraction) was initiated in Autumn 2016 following the TeaComposition protocols, in the same vegetation types. This series was fulfilled by the same amount and types of litter in 2017 and 2018, to follow the inter-annual dynamics of decomposition. We intend to initiate these series for 5 years to cover inter-annual dynamics. Also, in 2018 we have buried an enlarge series of native litters of 3 plants for 10 extraction years, which would allow to observe decomposition dynamics for longer time compared to short (4 years) period. The native litter types: Pinus sibirica needles, Betula pubescens leaves, Populus tremula leaves (coniferous forest); Scheuchzeria palustre leaves, Eriophorum vaginatum leaves, Carex limosa leaves, Sphagnum balticum upper parts (bog hollow); Ledum palustre leaves, Chamaedaphne calyculata leaves, Rubus chamaemorus leaves, Sphagnum fuscum upper parts (treed bog).
  • Another series of decomposition studies was initiated in 2016 in experimental conditions of temperature manipulation (raise for 1-2 degrees Celsius) in Open Top Chambers. The standard litter types (green, rooibos) were used with corresponding protocol of TeaComposition project. The litter bags were buried in two communities types, in OTC and control conditions, for 4 extraction years.
  • The standard litters decomposition studies were expanded to cover different forest types. Another series of plots was initiated in 2017 in vicinities of Shapsha field station, in after-cut succession forest types. Three types of forests (fresh cut, secondary aspen and old growth coniferous) were chosen to located 3 plots with 2 replicas each, for 4 extraction years. Following the protocol of TeaComposition project.
  • The standard (tea) substrate was also installed in 2019 to repeat the started experiments and to estimate local climate conditions in three native plots in Mukhrino, three plots in Shapsha and in OTC experiment. Following the protocol of TeaComposition, but only for 3 months decomposition period.
  • The standard (tea) substrate was installed in 5 ecotone transects in 2019 (from open bog to treed bog) to supplement the project TREEPEAT (group leader Joshua Ratcliffe). Following the protocol of TeaComposition, but only for 3 months decomposition period

You are welcome to visit a photoalbum on Flickr for pictures of plots, installation and extraction process.

An illustration of a global scientific initiative, TeaComposition, using #teabags to investigate leaf-litter #carbon gains and losses across the world.:

Last update: 25.02.2020